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These mutants are functionally normal in ciliogenesis at permissive temperature (18°C) but defective in anterograde IFT at non-permissive temperature (32°C).
This indicates that in the absence of MK-2206, AktW80A mutants are functionally equivalent to their WT counterparts.
eat-2 encodes an acetylcholine receptor subunit specific to the MC → muscle synapse; thus the mutants are functionally MC-minus (McKay et al., 2004).
Thus, we believe that the VF depots in these mutants are functionally distinct from the same fat depots in N mice and are beneficial, rather than detrimental, to the overall health of these mutant mice.
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On the other hand, the C197 mutants were functionally almost identical to wild-type BAS, excluding the possibility that the second active-site Cys is involved in the enzyme reaction.
The NO insensitive phenotype of the ahk5 mutants was functionally complemented by the wild type GFP-AHK5 construct (Fig. S1).
The mutants were functionally evaluated using the plate based dual complementation-cum-toxicity assay.
To determine whether transmission in sec8 mutants is functionally abnormal, we used voltage clamp synaptic electrophysiology on NMJs.
Therefore, if the SNP makes cdsA defective, the small cell size of the mutants is functionally relevant.
Based on these analyses, one could expect that the presence of CK1e mutants is functionally equivalent to the pharmacological inhibition of CK1e.
This ΔhtsAΔisdE mutant is functionally inactivated for both Isd and Hts-mediated heme transport.
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