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Note that E2F1 mutants are expressed at a higher concentration than the wild type.
The M5 and M9 mutants are expressed as both larger and smaller forms consistent with the partial proteolytic cleavage of their ECDs, as occurs with the wildtype Trask.
The trends can be better observed in Figures 3 and 4. In Figure 3, the rates of the L_K169, M_K173, and N_K158 mutants are expressed as a percentage of the wild type rate.
The previous studies had shown that the truncated M5 proteins encoded by these mutants are expressed in normal amounts on the S. pyogenes surface and have the expected binding properties (Figure 1B).
Despite evidence that NRPD1 and NRPE1/NRPD1b active site mutants are expressed at the same levels as non-mutant recombinant proteins and are not impaired in their ability to assemble with the NRPD2 subunit, the active site mutants fail to display the characteristic punctate nucleoplasmic localization patterns typical of wild-type NRPD1 or NRPE1.
When the mutants are expressed telomeres fail to lengthen.
Similar(39)
RAM GFP and PY mutants were expressed in HeLa cells resulting in equivalent expression.
The wild-type cueO gene and mutants were expressed in E. coli BL21 (DE3) using the pET expression system.
Mutants were expressed and purified as for the wild-type protein.
All hAID mutants were expressed and purified in the same way as wt hAID.
Twenty-three double mutants and twenty-two single mutants were expressed by Pichia pastoris.
More suggestions(16)
genes are expressed
transformations are expressed
ones are expressed
mutants are used
mutants are summarized
mutants are derived
mutants are described
mutants are affected
mutants are generated
mutants are shown
mutants are listed
mutants are obtained
mutants are born
mutants are found
mutants are reported
mutants are seeded
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