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Bmp and Wnt signaling were also found to be upregulated in incisor regions of Wise mutants and which we also observed in Lrp4 mutants [41].

Semi-thin sections of all embryos were first cut and stained with toluidine blue solution (0.2% toluidine blue O and 1% sodium chloride, pH 2.3) to identify which embryos are mutants and which embryos are controls, and to find the region where the embryo is located.

Finally, sgn3 does not show any of the enhanced suberin formation that has been observed in other mutants and which confounds interpretation of phenotypes.

Cluster 1 contains genes that were downregulated in injured nerves at 10 days post-injury in both control and conNrg1 mutants, and which tended to return towards baseline levels at 28 days.

To determine whether these are all false positives or marginally up-regulated genes, or rather, represent a class of genes up-regulated in the compatible interaction, a set of primer pairs was designed based on sequences of nine clones representing transcripts that were not identified [ 17] in the interactions with Col-0 and its mutants, and which have annotated functions (Table 2).

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We identified a mutation in the morning glory homolog of SHORT-ROOT (PnSHR1) that was genetically linked to the agravitropic phenotype of we2 mutant, and which may underlie the abnormal differentiation of endodermal cells in this plant.

However, fitness contours are not fixed but a function of the resident traits and we only consider those that divide the trait space into potentially successful mutants and mutants which are not able to invade ('invasion boundaries').

Similarly, we made RIG-I deletion mutants and determined which domain is required for interaction with REUL.

Therefore, we decided to further characterize the remaining 8 mutants and swi3-NBT7, wasch was individually isolated as a mating-type switching defective mutant (see Materials and Methods).

Fraction of animals exhibiting CL in let-60 lf) let-60 lfnd mutants in which the wild-type let-60 gene wandexpressed in AWC olfactory neurons and AIY interneurons.

The same number of copies was estimated for FL gene for Ugni Blanc mutant and Chardonnay which is heterozygous in this region.

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