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We observe variable effects on endoderm gene expression in aldh1a2um22 mutants and we therefore compared the aldh1a2um22 phenotype to the aldh1a2i26 phenotype.
Our results suggested that known miRNA targets fail to explain transcription patterns amongst miRNA mutants, and we devised a method to identify both novel miRNAs and miRNA targets.
While the microtubules in the mutants appear slightly more disorganized, variation between different embryos is seen in the microtubule organization in both wild-type and mutants, and we observed no gross defects in the microtubules (Fig. 2E).
We are interested in several receptor mutants and we implement them in different ways.
We transfected MCF-7 cells with WT HA-PTEN or the PTEN SUMOylation mutants and we performed immunofluorescent staining for PTEN using anti-HA antibody.
We knew a lot about mouse mutants and the genetic linkage map of the mutants, and we tried to fuse the mutant linkage map with the molecular map.
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A swordfish is basically a mutant, and we have no idea yet why it's got that long serrated thing sticking out of its face.
The increase in prospero transcript was larger in the bantam mutant, and we observed a change in Prospero protein expression in the mutant INP cells.
Cells were stably transfected either with the wild-type protein or with a kinase-dead (KD) mutant, and we found most DMPK in the mitochondrial fraction.
This construct (35S: TcNPR1:EGFP) was stably transformed into the npr1-2 mutand and we observed the localization of EGFP fusion protein before and 24 hrs after SA treatment in both leaf and root cells of four independent transgenic lines.
We have followed our reviewer's advice: we state that we study npr-1 null) npr-1 nulld that we refer to these as " npr-1" throughout.
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