Using ketosteroid isomerase as a model system, we correlate the field sensed at the bond involved in enzymatic catalysis with the rate of the reaction it catalyzes, including variations in this rate in a series of mutants and variants using non-canonical amino acids.
As well as naturally occurring mutants and variants, collections of insertional, ethylmethane sulfonate (EMS) and fast neutron mutants are available, particularly for tomato.
This Genome Database includes murine data on gene characterization, nomenclature, mapping, gene homologies, sequence links, phenotypes, allelic variants and mutants, and strain data [55].
The 'Mouse Genome Database' (1) includes data on gene characterization, nomenclature, mapping, gene homologies among mammals, sequence links, phenotypes, disease models, allelic variants and mutants and strain data.
For EGFR, ALK, and MET, truncated kinase domain structures have been determined many times, uncomplexed, in complexes with ATP, ATP analogs, and inhibitors, including mutants and truncation variants.
The majority of mutants and chromosome variants described in this review can be obtained at the Stock Center.
We conclude that phenotypic suppression in EMS-treated cpr1 mutants and bal variants is not limited to M1 plants with a chimeric morphology.
RNAi allows sequence-specific design to compensate for viral mutants and natural variants, thereby drastically expanding the number of therapeutic targets beyond the capabilities of cART.
We have recently performed a thorough characterization of thermal denaturation of holo- and apoproteins by differential scanning calorimetry in AGT WT and eleven mutants and polymorphic variants found in PH1 patients [ 48, 57].
For the WT, mutants, and polymorphic variants, docking against the ensemble of molecular dynamics conformations identified native-like binding poses of ATP close to the previously reported crystal structure of the ATP-bound state.
The transient expression system coupled with flow cytometry to detect intracellular levels of FVIII-based assay would be very useful in studying the intracellular trafficking or wild-type proteins as well as mutants and polymorphic variants of the protein.
