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The main exception to this is the EBNA3A mutants, two of which cluster with the mutants and two on the edge of the wild-type group.
To characterize the interaction between Mieap and NIX, two Mieap mutants and two NIX mutants were generated (Figure 3A and 3B).
To test the performance of the qTags we constructed three knockdown mutants and two reference strains and characterized their growth in three different media, their survival during oxidative stress, acid stress and starvation and their growth and survival in mice.
The GABA-T gene was sequenced from ten plants that phenotypically resembled pop2 mutants, and two new pop2 mutants with single point mutations were identified designated pop2-6 and pop2-7 (Figure 3A).
Nevertheless, even after outcrosses to minimize genetic background differences between mutants and two different revertant strains (bearing excision chromosomes lacking the P element), we observed small, albeit statistically significant, effects of the E74BG01805 mutation on the activity rhythm.
Cells then were transfected either with V5-tagged lipin-1α or lipin-1β, or with the corresponding double sumoylation site mutants, and two days later the subcellular distribution of the lipin-1 isoforms were detected by immunofluorescence with anti-V5 antibody.
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Twenty-three double mutants and twenty-two single mutants were expressed by Pichia pastoris.
To further map the gene, 50 F2 mutants and six polymorphic markers around RAL1 gene were used.
In addition, a number of single point mutants and three designed sequences are examined.
Eventually, four pairs of BRIL mutants and three pairs of Flavodoxin mutants, together with their WT proteins, were crystallized and structure determined (Tables S4 and S5).
To further map the gene, linkage analysis was carried out by using 50 F2 mutants and six polymorphic markers.
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