PIC1/Tic21 was proposed to form a protein translocation channel in the Tic complex, mainly based on protein import defects of knockout mutants and on structural similarities to amino acid transporters and sugar permeases [ 27].
However, detailed atomic-resolution structures of these mutants and the corresponding structural correlation of the substituted residue with its neighboring residues and the whole virus capsid have not been reported.
dys-1 mutants also exhibited morphological and structural defects.
Currently, specific transcriptional programs or cell phenotypes cannot be correlated with selected missense p53 mutations or even with the broad distinction between contact and structural mutants (39) (Table 1).
While mutations in a single real CAD gene rarely affect growth of transgenic plants, some natural mutants or double mutants have shown abnormal developmental and structural phenotypes.
To assess Mena localization in the postsynaptic space, we expressed wild-type Mena (UAS-EGFP-Mena WT) and structural mutant transgenes using the pan-mesodermal how 24B-Gal4 driver, and immunostained with anti-EGFP and anti-Dlg antibodies.
To delineate the difference, we designed 13 BRCA1-BRCT mutands and computationally investigated the structural and stability changes compared to the experimental p53-53BP1 structure.
To test whether Salmonella flagella have a function in competitive persistence within tomato fruits, phenotypes of regulatory and structural flagellar mutants were compared.
However, these mutants (which perturbed both electrostatic and structural properties of the native VSD) significantly altered closed state inactivation (CSI) and recovery from both open and closed inactivated states, effects that cannot be accounted for by the conventional Shaker model [11] [13].
Decreased ERK activation as we observed in mutants might ensure neuronal resistance and structural plasticity, thereby facilitating cell integration.
