Sentence examples for mutants and selection from inspiring English sources

Different display systems that allow construction of libraries of mutants and selection of binders with improved affinity, along with the gene coding for these variants, have been developed such as phage display or ribosome display systems (Hanes and Plückthun 1997; Bratkovic 2010).

To verify the degradation of the CL1 degron by autophagy, we generated a P. anserina strain lacking PaAtg1 and expressing Gfp-cl1 by crossing of single mutants and selection of the double mutant.

PaGlo1/ PaGlo2_OEx double mutants were generated by crossing the single overexpression mutants and selection of progeny in which recombination resulted in the combination of the PaGlo1 and PaGlo2 overexpression cassettes in one nucleus.

More than 250 plasmids were assessed by re-transformation of the mutant and selection for thermoresistance before analysis with restriction enzymes and sequencing.

Open field day will be held each year for public touring the mutant plots and selection of interested mutants.

Besides, keeping the lethal rate high is necessary for the effective mutation and selection of mutant strains.

Nevertheless, maintenance of large mutant libraries and selection methods are difficult to implement in more complex systems such as mammalian cells and multicellular organisms.

Mutation induction and selection of mutants have been powerful tools for plant breeding as well as for physiological and molecular studies for the past 80 years.

In "first-generation" msh1 mutants created by pollination of a wild-type line with mutant msh1 pollen and selection of the F2 msegregantsegregant, low-level recombination activity is initiated at each repeat.

Additional kanamycin was then added to bioreactors to concentrations typically used for mutant and plasmid selection (200 μg/ml), as well as a concentration representing only 25% of this level.

The HeLa-tTA subline was subjected to a second round of transfection with constitutively active mutant GTPase plasmids and selection with puromycin, zeocin and dox.