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Starchless mutants that stained pale green instead of purple were considered as potential starchless mutants and selected for further study.
Colonies that did not stain purple after the iodine vapour treatment were considered as potential starchless mutants, and selected for further analysis.
For this, we infected zebrafish embryos with individual M. marinum transposon mutants and selected for mutants that were attenuated for bacterial aggregation as compared with wildtype.
In this work, we aimed to further characterize malfunctioning mechanisms of HMBS mutants, and selected four natural mutations found in Norwegian AIP patients (R116W, R167W, R173W and V215E).
The IL-3-dependent murine cell line 32D was transfected by retroviral vectors expressing WT KIT or 1 of 17 KIT mutants and selected for IL-3-independent growth.
The aim of our study was to (1) characterize the shoot and root system of GA biosynthesis mutants and selected arabidopsis accessions carrying functional and mutated alleles of the GA5 gene, and (2) to evaluate their response to reduced water availability.
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The various plasmids were transformed into the katG deletion mutant and selected on Luria agar containing hygromycin (50 µg/mL).
For the rescue experiments, HA-tagged VAMP8-expressing cells were further transduced with constructs expressing myc-tagged siRNA-resistant CALM (both wild-type and mutant), and selected with 0.2 mg/ml hygromycin B together with 0.5 mg/ml G418.
We screened a collection of yeast kinase gene deletions consisting of 121 mutant strains and selected 21 kinase mutants that grew better than WT cells in medium containing 0.8 M NaCl.
To help avoid potential modifiers of mating behavior from the Hawaii strain, we intragressed the CB4856 sequence into the lon-2 egl-15 interval of N2 by crossing CB4856 with the lon-2 egl-15 double mutant and selecting a non-Lon non-Egl F3 population (three times).
To further delete hrd1+ from scp1+ WT and mutants (M1-M3), hrd1Δ strain PEY1756 was mated to scp1+ WT and mutants (M1-M3), respectively, and selected on medium containing both clonNAT (100 μg/l) and Geneticin (100 μg/l).
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