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Mice were viable at P1 and OPC cultures were generated from mutants and normal littermates as previously described.
This method allows the identification of statistically significant overlap or discrepancy between the gene signatures across the different mutants and normal signalling.
Genes highly connected in the network are hub genes, which tend to exhibit higher expression changes between rx3-/ mutants and normal phenotype siblings.
A comparison of meiotic mutants and normal genotypes leads to the conclusion that the genomic distribution of crossover exchanges in normal meiosis is more restricted and less proportional to physical distances than in meiosis altered by mutations [ 64– 64].
Evidence is accumulating, based on murine models (e.g., Laron dwarf mutants and normal mice under CR), for an association between low-IGF-1 signaling and extended longevity [ 3- 4].
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But you can't say mutant and normal cells respond to the chemical differently: to say that, you would have to do a third statistical test, specifically comparing the "difference in differences", the difference between the chemical-induced change in firing rate for the normal cells against the chemical-induced change in the mutant cells.
Under normal conditions, both mutant and normal mice are moderate tipplers, drinking some alcohol but choosing pure water most of the time.
By comparing the genetic sequences of the mutant and normal flies, the researchers found that the forgetful flies had an inactive copy of a gene that he and his Chilean collaborators dubbed Volado (Vol --South American slang for absentminded.
There was no difference between mutant and normal mice when it came to investigating an empty cage, however, and the mutants even outperformed normal mice on a test of spatial learning and memory, suggesting that their deficit was specific for social behavior.
To fine map YGL138 t) gene, a large F2 population derived from the cross of ygl138 mutant and normal green leaf variety Minghui 63 (Oryza sativa L. ssp. indica) was constructed, and 836 recessive individuals with yellow-green leaf phenotype in the population were selected for fine mapping.
Attempts at siRNA knockdown of mutant TTK were hindered by difficulty in resolving the mutant and normal protein bands.
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