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Only two of ten nitrofurantoin-resistant mutants and four of ten trimethoprim-resistant mutants displayed an apparent cost of resistance.
Cohesion analyses were repeated three times for wildtype cells, two times for elg1 mutants and four times each for ctf7eco1-1 and ctf7eco1-1 elg1 mutant cells with a minimum of at least 100 cells scored in each trial.
Each group contained four mutants and four littermate controls.
Quantification of phospho-SMAD3 (pSMand) and αSMA immunofluorescent staining was determined using stained cryosections from four mutants and four controls at E11.5.
These mice served as the founding strains for the Peromyscus Genetic Stock Center PGSCC) established in 1985 by Wallace Dawson at the University of South Carolina, which currently maintains wild-derived stocks of six species, as well as 13 coat-color mutants and four additional mutants on P. maniculatus genetic backgrounds.
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Twenty-three double mutants and twenty-two single mutants were expressed by Pichia pastoris.
In addition, a number of single point mutants and three designed sequences are examined.
Three mutant SSUs were obtained from spontaneous ValR bacterial mutants and three more were designed on the basis of an alignment of SSU sequences from valine-sensitive and resistant isozymes, or consideration of the molecular model developed here.
To further map the gene, 50 F2 mutants and six polymorphic markers around RAL1 gene were used.
Eventually, four pairs of BRIL mutants and three pairs of Flavodoxin mutants, together with their WT proteins, were crystallized and structure determined (Tables S4 and S5).
To further map the gene, linkage analysis was carried out by using 50 F2 mutants and six polymorphic markers.
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