We then created tt2-5 sk11 sk12 triple mutants, and found that tt2-5 completely suppressed sk11 sk12 phenotypes pertaining to seed coat color (Fig. 3f) and fatty acid levels (Fig. 3g, left panel).
We constructed MC159 truncation mutants and found that the amino-terminal region before the first DED and nearly all the carboxyl terminus after the second DED were dispensable for the antiapoptotic activity of MC159.
Using an assay developed by us previously21, we quantified the frequency of emergence of phage resistant mutants, and found that growth in LB supplemented with spent medium containing autoinducers caused the emergence of phage resistant derivatives of the target bacterium at an enhanced rate as compared to a culture without added autoinducer preparations (Fig. 4).
Having established that ephrinB2 can associate with TBC1d24, we expressed TBC1d24 in the embryos along with ephrinB2 deletion mutants and found that deletion of six amino acids in the C-terminus encompassing the PDZ-binding motif markedly reduced the interaction with TBC1d24 (Fig. 1b).
No significant alteration in the contents of xylose and lignin were detected (Additional file 4: Table S1) We determined the expression levels of OsMYB61 and secondary wall CESA genes in two mutants and found that the expression levels of OsMYB61 and CESA genes were down-regulated (Fig. 5f).
We introduced Pglr-1::rpm-1 into rpm-1 mutants, and found that it rescued (Fig. 1H,M).
Cortez et al. investigated the Y177F and SH2 mutants and found they both were able to transphosphorylate an ABL kinase substrate at similar levels to wild type [28].
We have characterized the growth phenotypes and the polyhedral microcompartment-like structures formed in these mutants and found that carboxysome shell formation is independent of RubisCO sequestration.
Finally, we examined several eating-defective mutants and found that both eat-2 and eat-5 mutants stored lower fat on both bacteria (Figure 5C).
We analyzed germ line clones of 14 loss-of-function mutants and found 8 genes that showed a similar phenotype to that seen in Dg loss-of-function clones.
We also generated uev-1 ubc-1 double mutants and found that they were viable and had similar GLR-1 trafficking defects to uev-1 single mutant animals (data not shown).
