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The importance of accurate estimation of the cell growth rate is more eminent for the dynamic simulation of the specific gene knockout mutants and for the effect of culture condition.
Over a year and a half, this small but dedicated group grew 350,000 yeast colonies, picked out and cultured 35,000 promising mutants, and, for a selected subset of those, analyzed their telomere lengths.
For each of these mutants and for wild type cells we established clones expressing CFP-ubiquitin, mRFP-PCNA or both.
This MAb exhibited only minor reactivity reductions (50 25%) for all six G106/L107 fusion peptide mutants and for two individual EDIII mutants E311R and P364R (50%).
Indeed, the absence of RND components AcrB or TolC in Salmonella enterica caused widespread repression of chemotaxis and motility genes in these mutants, and for acrB mutant this was associated with decreased motility [45].
Their offspring until F5 were crossbred again and genotyped for E- and P-selectin-null mutants and for scid.
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N = 22 worms for wild type, 27 for nlp-24 mutants, and 19 for nlp-24 rescued worms.
The extinction coefficient was 23970 cm−1 M−1 for triple tryptophan mutants, 29660 cm−1 M−1 for double tryptophan mutants, and 41040 cm−1 M−1 for wild-type HγD-Crys.
We expressed these mutant visual pigments using SRα for the PrB mutants and pcDNA3.1 for the PrV mutants.
We thank Steve Rice for providing viral mutants, and Ernst Ungewickell and Daniel Manor for Hsc70 plasmids.
For this, we infected zebrafish embryos with individual M. marinum transposon mutants and selected for mutants that were attenuated for bacterial aggregation as compared with wildtype.
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