Others produced a SAGE library of transcripts from dissected worm intestines from mutant adults, and detected a total of 5,623 intestine genes [ 43].
The HA-tagged Mitf-A and its mutants were expressed and detected by α-HA Ab (Fig. 2a).
We sequenced the sec23 genomic DNA of embryos homozygous mutant for hau and detected a point mutation in each of our alleles (Figure 11B).
We determined the solution structure of the I214V mutant of RaPrPC 91 228) and detected the backbone dynamics of its structured C-terminal domain (121 228).
To assess neuronal loss, we quantified DAC-positive neurons in puc mutant and control embryos and detected a significantly lower number in the mutants.
The Cys28 in the RING domain of RAD18 is the key site essential for its E3 ligase activity (Huang et al., 2009b), we constructed two RAD18 mutants, C28F and ΔRING, and detected their effects on PCNA NEDDylation.
To exclude that this could depend on defective neurogenesis from the SVZ, we counted the number of PDGFR α+ cells in the SVZ of mutant and wt mice and detected no differences.
To determine the unselected formation rate of mDEL1 mutations using mutants detected selectively, the starting tester strain carried a purR mutation and mutants were detected as colonies appearing on day 2.
Yet, for some of them, exemplified by the novel pervasive transcripts found antisense to MAL12/32 and shown in Figure 4E, there is a strong signal in the double mutant and no detected signal in the single mutants.
Mutants of a C-terminal Ser residue, (S141A and S141P), tri- or tetra-CQES repeat mutants, and CRS mutants were detected as wild-type CST1, and phosphorylation of the Ser residue was not detected in our study.
