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To evaluate whether PsAvr3c contributes to P. sojae virulence in the absence of Rps3c, we compared the growth of the PsAvr3c mutants and control strain on susceptible soybean plants.
To allow for the readily comparison between mutants and control mice we normalized the values of the SS spiking produced by the model to the average SS frequency of the control cells (Fig. 2f).
Quantification of ipsilateral projections between different mutants and control mice reveals comparable proportions of ipsilateral projections.
At this stage mutant livers were hypoplastic, whereas E12.5 mutants and control livers showed comparable histology (Fig. S4B to E).
We found tight conservation of the total and relative expression of all three NOS isoforms in the NOS2 mutant, with protein ratios between mutants and control neocortex measuring 88±16% for NOS1, 106±17% for NOS2, and 103±17% for NOS3 (p>0.05, n = 6 animals of each strain, Fig 6A).
In order to compare the velocities of heart wall motion between TM23 mutants and control larvae, only the part of the tracing showing the highest frequency within a heartbeat (boxed in Fig. 3A, 3B, bottom panels) was used in calculating the velocity (as described in Materials and Methods).
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The selected normalization factor of 60.05 Hz was kept constant for all mutants and controls.
However, after 24 h, both mutants and controls reached nearly the same angle of curvature.
At P1 and P5, there were no apparent differences between mutants and controls (data not shown).
The Mann-Whitney U test was applied for comparisons between mutants and controls.
Under paraquat-induced stress, the difference in oxidation between mutants and controls increased markedly.
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