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Indeed it is shown that the temperature-sensitive cut11.1 mutant has monopolar spindles with asymmetric SPB defects, as in the cut12.1 or pcp1 mutants analysed here (West et al, 1998; Tallada et al, 2009).
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In addition to the three disease-related β-actin mutant analysed here, more than sixty α-skeletal muscle actin mutants that cause nemaline myopathy have been isolated (5), four α-cardiac actin mutants that cause cardiomyopathy (55, 56) and recently five γ-actin mutants that are associated with dominant progressive deafness (DFNA20/26) (57, 58).
The Ptbp1 mutant mouse generated and analysed here demonstrates how the absence of an RNA-binding protein, involved in splicing and RNA stability among other functions, can completely halt embryonic development.
Recall that mutant tai animals indeed had enlarged niches and extra GSCs, a phenotype not seen in other cases analysed here.
A sum that has been deconstructed and analysed here, here, and here.
Similarly, mutants of the laboratory constructed recombinant genome, VWMPCPLIRMat [ 51] are also mentioned here for the first time, whereas mutated, mostly MSV-MatA-like, recombinant genomes (see Additional files 3, 4 and 5), have been reported previously [ 47] and are re-analysed here.
The Tdg KO strategy, cell culture conditions and in-vitro differentiation procedure used to generate the 18 wt and mutant samples analysed here were as described in Cortázar et al. (2011) [ 13]. 5 μg of DNA from each sample was sonicated to between 50 and 350 bp.
We provide results from both analyses here.
Each mutant was analysed in triplicate.
Ten cells of each mutant were analysed.
Here, we performed expression and mutant analyses to identify the upstream regulator of miR156.
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