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Multilayering of the FE has been reported for mutants affecting the apicobasal polarity of the follicle cells themselves.
The importance of these domains has been demonstrated by the genetic characterization of several A. nidulans mutants affecting the sphingolipid biosynthesis pathway.
Aberrant axon growth into the contralateral optic nerve similar to what we detected in our Dicer mutant mice, has been reported in Slit1/Slit2 double mutant mice [63] and in mutants affecting the heparan sulfate proteoglycan sulfation pathway [64], [65].
We also analysed the formation of ChP in mutants affecting the Notch and Hh signalling pathways and defined specific developmental defects of ChP caused by genetic abnormalities affecting these pathways.
We compared their viability to egr mutants and other mutants affecting the Toll signaling pathway, spz and PGRP-SA, which contribute to the resistance to systemic gram-positive cocci infection [18], [19].
Because initial binding is independent of codon:anticodon interactions, mutants affecting the first step would be expected to alter fidelity in response to both near- and non-cognate codons.
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Because distinct mutants of PML-RARA exhibited different AS2O3 binding affinity and degradation pattern followed by AS2O3 treatment, we next detected whether these mutants affect the basal SUMOylation of PML-RARA.
None of the mutants affected the relative amplitudes of the fast and slow components of deactivation at the most negative potentials.
To examine if the remaining mutants affected the outer layers of the spore wall, dityrosine fluorescence of the spores was examined (Figure 5).
Another assay to test the activity of the Ser103 mutants is to investigate whether these mutants affect the dominant BicD phenotype.
Since Wingless and ArmS10 signalling caused an apparent dissociation of E-Cadherin and Armadillo, we wanted to examine how these Armadillo mutants affected the distribution of E-Cadherin.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com