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Morrison, M., Harris, K.S. & Roth, M.B. smg mutants affect the expression of alternatively spliced SR protein mRNAs in Caenorhabditis elegans.
We conclude that the five VV mutants affect the 132-kDa subunit of the VV RNA polymerase.
Because distinct mutants of PML-RARA exhibited different AS2O3 binding affinity and degradation pattern followed by AS2O3 treatment, we next detected whether these mutants affect the basal SUMOylation of PML-RARA.
Using circular dichroism, we find that 2 mutants affect the alpha-helical coiled-coil structure of individual molecules, and 2 mutants disrupt the lateral associations among individual molecules necessary to form higher-order assemblies, helping explain the dominant effects of these mutants.
Another assay to test the activity of the Ser103 mutants is to investigate whether these mutants affect the dominant BicD phenotype.
Previous studies indicated that sterols, including typical sterols (sitosterol and stigmasterol) and atypical sterols (8, 14-diene sterols accumulated in fk mutants), affect the expression of genes involved in cell expansion and cell division [27].
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A simple mathematical model of this mechanism correctly predicts how the wild-type expression pattern of tll changes in mutants affecting the anterior, dorsoventral, and terminal patterning systems and some of their direct targets.
Multilayering of the FE has been reported for mutants affecting the apicobasal polarity of the follicle cells themselves.
None of the mutants affected the relative amplitudes of the fast and slow components of deactivation at the most negative potentials.
To examine if the remaining mutants affected the outer layers of the spore wall, dityrosine fluorescence of the spores was examined (Figure 5).
The importance of these domains has been demonstrated by the genetic characterization of several A. nidulans mutants affecting the sphingolipid biosynthesis pathway.
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Justyna Jupowicz-Kozak
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