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C. neoformans ssn8 mutants accumulate more melanin than the wild-type strain (Fig. 8A).
The assumption that both TAG synthesis and lipolysis are simultaneously active processes in proliferating cells is supported by the observation that lipase-deficient yeast mutants accumulate more TAG than WT cells at any given time point, without significant impact on cell size or growth rate.
For example, many genes in the BIN "Anthocyanin metabolism" were up-regulated in npq4, in accordance with our previous finding that under many conditions npq4 mutants accumulate more anthocyanins than wild-type plants [ 5], but the BIN as a whole was not significantly up-regulated.
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Seeds of GmMYB73-overexpressing plants and gl2 mutant accumulated more lipids and fatty acids.
In PCC 7119, a susB mutant had no effect on growth or sucrose production [ 91], whereas a susA mutant accumulated more sucrose and less glycogen than the wild-type strain in N2-fixing conditions [ 91, 97, 98].
Interestingly, an ahp mutant in a catalase-deficient E. coli mutant accumulated more H2O2 intracellularly indicating a primary role for Ahp in scavenging intracellular H2O2 (Seaver and Imlay 2001).
The mtsR mutant accumulated more intracellular iron compared to wild-type in Fe uptake assays in complete medium, and was hypersensitive to H2O2, indicating that MtsR plays a role in GAS resistance to oxidative stress (Bates et al., 2005).
The Δ htrA/hhoA/hhoB triple mutant accumulated more oxidatively damaged proteins than the did the wild-type, did not grow at high-intensity light or high temperature and showed severe deficits in phototaxis [ 29].
Similarly, although osu1 had no difference in anthocyanin levels under 15C/4N compared to WT, reducing N from 4 to 1 mM caused osu1 mutants to accumulate more anthocyanins while WT did not.
For example, under 15C/4N (a C∶N ratio of close to 4), osu1 and WT seedlings had similar root lengths and anthocyanin levels (Figure 2), but 120C/30N ( a ratio of 4) led osu1 mutants to accumulate more anthocyanins than WT (Figure 1D).
Conversely, atips1 mutants did not accumulate more starch than the wild-type and 31P-RMN analyses did not reveal any modifications in pools of Calvin cycle intermediates such as ribulose-1,5-bisphosphate (data not shown).
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