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The lac+ strains were categorized into the early- and late-arising mutants according to the time for colony appearance.
FMTS only generated valid mutants according to FaMa.
We obtained wild-type, heterozygous, and homozygous mutants according to the Mendelian ratio (69 +/+; 137 +/−; 64 −/−).
Larvae at 3 4 dpf were anesthetized with 3-aminobenzoic acid ethyl esther methane sulfonate (MESAB) to sort the homozygous mutants according to their eye pigmentation phenotype [1].
Neither DNA nor RNA had any effect on both Aburst and kburst for the N- or I-DDX3 mutants, according to their loss of stimulation by NA.
We have previously obtained a preliminary model in order to explore the possible effects of FOXL2 disease-causing mutants according to their predicted structural positioning [26].
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Unique primers are designed for each Sav mutant according to the guidelines summarized in Box 1 (ref. 32).
Deadpool will be the next X-Men character to get his own spin-off movie, with Ryan Reynolds likely to get a second shot at playing the unpredictable self-healing mutant, according to the Hollywood Reporter.
The psaA gene has been shown to be positively regulated by RovA [6], and ompC, which has been previously shown to be associated with membrane permeability, was down-regulated by >20 fold in the ΔrovA mutant according to the microarray analysis.
Only a few results differed from those obtained from transfected HEK293 cells: First, normalized peak currents obtained from oocytes were significantly smaller for E161K, D1275N and even for P1298L, which was classified as a group 1 mutant according to results from HEK293 cells (Figs. 1 and 4).
Approximately (3+), (2+), (1+), and represented more than 20, 2 20, 0.2 2, less than 0.2% and none (not detected) of the mutant, according to the manufacturer's instructions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com