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Briefly, to isolate inx-8 inx-9 double mutants, a sem-3(n1655) inx-9 ok1502) inx-9 ok1502irstrainstructed.
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To isolate inx-8 inx-9 double mutants, sem-3 inx-9 males were mutagenized with EMS and crossed to dpy-20 hermaphrodites.
The mutant A(H5N1) strain has killed birds in Cambodia, China, Indonesia, Japan, Laos, South Korea, Thailand and Vietnam.
sem-3 mutant animals have defects in egg-laying muscles and retain most of the eggs they produce, resulting in fertile adults bloated with eggs that hatch inside the parent.
Candidate sem-3 inx-9 sterile mutants were examined for phenotypic resemblance to inx-14 tm2864) inx-14 tm2864ls produced) and fewther characterized.
We screened for candidates that phenocopied inx-14 tm2864) inx-14 tm2864y representing sem-3 inx-8 inx-9 mutasts.
However, the mutant embryos analyzed showed an average index of only 43.1% (with a SEM of 6.7%) (Figure 4A4 and 4B), suggesting a significantly impaired cell proliferation capacity in mutant embryos in vivo.
Coverslips were then sputtered with gold (Sem Coating System, Polarion) and SEM was carried out using a Philips SEM 505 (Eindhoven, The Netherlands).
The SEM images and corresponding EDX elemental mapping were recorded on an SEM (FEI XL30 Sirion) at an acceleration voltage of 5 kV.
SEM observations were conducted using a SEM S-5000 field-emission scanning electron microscope (Hitachi Science Systems, Tokyo, Japan).
SEM was performed to evaluate the surface morphology of NS using a SEM XL-30 (Philips, Eindhoven, the Netherlands).
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