Exact(1)
Of these, 268 ncRNAs showed reduced and 322 ncRNAs showed elevated expression in the cep-1 mutant worms when compared to the wild-type N2.
Similar(57)
Since we sampled all of our mutants at three ages we were also able to study, at least crudely, when the mutant worms acquired their distinctive metabolic profiles.
These mutants exhibited embryonic osmotic sensitivity when homozygous mutant worms were up-shifted to the restrictive temperature at the L4 larval stage, after the completion of most gonad development.
We compared the fat content between the wild-type and c30f12.4 mutant worms by fixed Nile Red staining when they were fed with the standard Escherichia coli OP50 diet ad libitum.
dnj-14 mutants are almost indistinguishable from wild-type worms when young, but have a reduced lifespan and develop profound sensory neuron defects with increasing age.
Moreover, upon UV treatment, when ∼20% of smc-5 (ok2421 ) mutant worms were still capable of forming disrupted germlines, all double-mutant worms completely lacked germlines.
Knockdown of three splicing factors, prp-17, lsm-4, and gut-2, resulted in enhanced sterility when depleted in rrf-1 ; fbf-1 mutant worms compared with the rrf-1 strain.
Interestingly, both mpk-1 mutant worms and germline deficient glp-1 mutants exhibit elevated baseline stress resistance when compared with wild type control animals., The stress resistance of glp-1 mutants is thought to reflect the general stress resistance that can be caused by a complete ablation of the reproductive germline.
Unlike normal worms, when the germline stem cells were removed from the mutants, their lifespan did not increase.
Indeed, one of the most exciting moments of this lab for the instructors was when a lab group related the egg-laying defect in brc-1 mutant worms to the role of its orthologue (brca-1) in human ovarian cancer.
With fewer bacterial crops to harvest, the population of mutant worms was smaller than that of the normal worms.
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