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Both mpk-1 and glp-1 mutant worms fail to upregulate the expression of innate immunity-associated genes upon genotoxic stress, indicating that MPK-1 activity in the germline is required for inducing innate immune genes.
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The number of DD axons in newly hatched L1 mutant worms that failed to reach the dorsal nerve cord increased by only 2% compared to wild-type controls, but 20% of mutant DD L1 axons (approximately 1 axon per animal) failed to extend their full distance along the dorsal cord (Table 2; Figure 3).
However, exposure of rec-8; chk-2 mutants to the dose of γ-irradiation that restored SCC in spo-11 rec-8 worms failed to restore SCC in rec-8; chk-2 mutants.
And indeed, while the worms in the control group were swimming and eating again at Day 8, these worms failed to grow or move.
Finally, pcs-1 tm1748) pcs-1 tm1748 to accumulate PC wormscultured in medium supplemented with Cd.
Plates on which the worms failed to grow and plates on which the worms had burrowed into the agar were excluded from the analysis.
Though expression pattern, sub-cellular localization and mutant analysis might suggest an important role for SMF-1 in Mn2+ uptake, Mn content measurements failed to show a significant difference between wild type and smf-1 eh5) smf-1 eh5rmutant
With fewer bacterial crops to harvest, the population of mutant worms was smaller than that of the normal worms.
Kenyon showed me one of her mutant worms moving across a microscope slide as gracefully and vigorously at eight weeks as a normal worm moves at two weeks.
On the glowing Perspex model, it looks like an army of mutant worms wriggling towards the power station, set to throttle it once and for all.
Such mutant worms can be bought off the shelf, whereas the ones with just an extra sir2 gene are a special order.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com