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An enzymatic dead mutant without the N-terminus also has tumor suppression activity (Nesiel-Nuttman et al., 2015).
Moreover, creation of a water channel to the heme center significantly enhanced the NIR activity compared to the corresponding mutant without the channel.
While the fusion protein localizes to the nucleus, its deletion mutant without the PHD domain resides exclusively in the nucleolus, suggesting a potential link between chromatin-binding PHD domain and nuclear architecture.
A mutant without the PP1-binding domain (Δ469 518) induced cell rounding or shrinkage instead of cell spreading.
We have also confirmed that the tetherin mutant without the GPI anchor showed complete loss of the antiviral activity (data not shown).
Overexpression of a Myosin 1E deletion mutant without the extreme C-terminal interacting (SH3) domain (Myosin 1EΔSH3) increases edge fluctuations and decreases stable adhesion lifetimes.
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Second, if their model is correct, the trimming of the outer layers of the peptidoglycan by sub-inhibitory concentrations of mutanolysin should abolish PGRP-SA binding to the atl mutant without affecting the biological activity of the released peptidoglycan.
We also retained mutants without the occurrence of root swelling after exposure to Al.
In conclusion, pHJOFpac is an ideal construct for the rapid production of VZV mutants without the retention of any unwanted operational sequences in the manipulated viral genome.
dla mutants do not have defects in Zn5+ cell number, but have similar aberrant patterning as in srn mutants, without the loss of the dorsal projecting nerve (Fig. 4A, B, dashed blue bracket and solid pink oval, respectively).
We furthermore surveyed if sequencing of only the mutants without the WT would have been already sufficient to identify the specific variants in each mutant.
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