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Methods A and B only required separation of the normalized ratios of the three possible genotypes (wild-type, heterozygote and mutant) within a certain difference (d) between the minimum and maximum ratios observed for each respective genotype.
The population of growth-deficient mutant within a mutant pool was examined by real-time PCR.
In contrast, the relative abundance of a mutant within a pool we consider less informative, since it may be influenced by a number of additional factors, such as the abundance of a vector in the transfection cocktail, the length of its homology arms, or any local variation in recombination rates.
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FgMutantDb aids in the maintenance and sharing of mutants within a research community.
In a set of experiments mimicking the occurrence of sporadic mutants within a sea of normal cells, Olariu et al. found that aneuploid hPSCs with representative commonly observed karyotypic changes quickly outcompeted their normal counterparts (Olariu et al., 2010).
However, if this number is substantially lower for the stochastic simulations for example, 25 (6) as compared with 10,000 (used for most of the figures)—the slower growth and lower total number of viruses could substantially delay the appearance of mutants within a host.
Our ability to identify specific point mutants within a complex population validated the assay's ability to reproducibly detect polymorphisms present at less than 1%.
Under the conditions of mutation-selection equilibrium, fitness interactions between mutations will affect the frequency of individual mutants within a virus population.
We tested the sensitivity and accuracy of the MDAP platform by measuring the frequency of specific mutants within a defined population.
Mutants within a complementation group exhibited similar frequencies of nondisjunction.
We found that UPTAGs and DNTAGs for each mutant had similar counts in the majority of samples, with 2574 mutants within a two-fold difference of each other.
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