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This mutant with two cysteine to serine mutations, HPTP-BNΔ6-CΔ2-C90S-C109S, has good potentian as an optimal reporter.
In the mutant with two symmetrical hydrogen bonds (FM197H) a single cooperative rupture of the two bonds was observed corresponding to an about twice stronger bond, rather than a sequential rupture of two individual bonds.
Consequently, SInv and 5 mutants including three mutants with single point substitution [Mut1 = P152 V, Mut2 = S85 V and Mut3 = K153F ], one mutant with two points [Mut4 = S305V-N463 V] and one mutant with three points [Mut5 = S85V-K153F-T271 V] were developed via site-directed mutagenesis and produced using Pichia pastoris as the host.
We also compared the Al resistance of ral1 mutant with two Al-resistant japonica cultivars NPB and Koshi, and the results showed that the mutant was slightly more resistant to Al than the Al-resistant cultivars (Fig. 2b).
To generate a mutant with two deleted lsp genes (i.e., Δlsp12, Δlsp23, or Δlsp13), the suicide vector pEX18GM containing one of the lsp genes was introduced into a mutant strain with a different lsp gene already deleted.
It is now a mutant with two right wings: one wing is voter ID suppression and the other wing is the school-to-prison pipeline for our children.
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Herein, a further increase in fluorescence in the new BFPvvD8 mutant, with three additional mutations from BFPvvD7, was made.
To this end, we generated one quadruple-point mutant with four threonine residues mutated to alanine (A) (referred to as Ezh2-M 4A), and one triple-point mutant with a T372 residue remaining intact (referred to as Ezh2-M 3A372T).
A C-cap mutant with three additional mutations introducing suitably spaced charged residues did not show formation of salt bridges, explaining why its stability was not increased further.
The walls were crowded with framed images, as at the Barnes Foundation, except in this case the collection featured Edward Gorey illustrations, concept-art sketches of the demon from "Fantasia" ("I'm an obsessive Disney-villain guy"), and comic-book panels, including a Richard Corben drawing of a mutant with four breasts.
A selected mutant with five substitution mutations scattered throughout the primary sequence showed greater catalytic activity than the original ribozyme under the selection conditions.
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