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Specific activity calculated from the level of total proteins is a favorable index for recognizing an enzyme mutant with small improvement of activity.
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Conversely, the krp4-2 mutant, with smaller meristem cells, formed more sepals and petals, and fewer stamens.
The uricase of Bacillus fastidious is a promising biodrug for treating refractory gout and a suitable tool for serum uric acid assay [32 34]; we cloned this uricase gene and obtained some mutants with small differences in catalytic capacities.
SV40 mutants with small deletions or base substitutions have been isolated by in vitro site-specific or random local mutagenesis of recombinant DNA followed by cloning in E. coli.
The log-fold change distributions of known miRNA targets were also very similar between mutants with large transcriptome changes and mutants with small transcriptome changes (Figure S2).
Surprisingly, however, many mutants with small birth size did not have a small size overall (ρ = 0.40; see Figure 3).
Many mutants with small birth size also had normal %G1 DNA content (see Figure 5, and File S1).
Specifically, mutants with small or large mean size were not significantly overrepresented in the group of mutants with a high G1 DNA content (ρ = −0.09).
In agreement with data from the D139A crystal structure, we identify a new water positions (water 15) for mutants with small side chains (D139A and D139N).
The general conclusion from the behavior of this system is that populations can respond rapidly to selection, when mutants with small improvements are common.
To illustrate such complexity, some mutants with small mean size (e.g., sfp1 Δ cells) may have a small birth size and a reduced critical threshold for initiation of division (Hoose et al. 2012).
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