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This implies that the mutant stabilizes the active more than the inactive conformation, consistent with a higher constitutive activity of the mutant with respect to the wild-type receptor.
We have characterized the mutant with respect to growth phenotype and to carboxysome composition and function.
The transcript level of TPK3 was slightly reduced in the UTR-insertion mutant with respect to that found in wild-type (not shown).
Our results demonstrate that dosR expression is reduced in the phoP mutant with respect to the wild type strain and, complementation of the phoP mutant with the phoPR operon restores dosR expression to wild type levels (Figure 3).
From the log ratios of expression levels of a mutant with respect to the wild type, we want to identify those subsets whose expressions are significantly up-regulated or down-regulated.
Array-CGH data are signal ratios of mutant with respect to wild type samples for each single EST.
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Molecular dynamics calculations and unfolding experiments highlighted a higher structural stability of these mutants with respect to the wild-type.
It would be interesting to investigate potential ATA resistant mutants with respect to NA amino acids mutations.
Notably, there was an internal consistency with respect to percentage activity reduction comparing individual mutants with respect to autophosphorylation and phosphorylation of Cdc25 (Figure 3).
In fact, the triple mutant strains were indistinguishable from the fat-6;fat-7 double mutants with respect to fat stores, growth rate, and brood size.
To address these questions, we investigated 14 LPS B. melitensis mutants with respect to LPS defects, intracellular multiplication and virulence and vaccine efficacy in mice.
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