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The mutant, with improved metal binding abilities, has been immobilized using glutaraldehyde on PAMAM dendrimer-modified magnetite nanoparticles.
The results of this study should be important for the rational design of a DehL mutant with improved catalytic efficiency.
This enzyme was subjected to directed evolution experiments that allowed developing a mutant with improved activities (Giacobbe et al. 2014) and effect in biomass conversions (Marcolongo et al. 2014).
The importance of this oligomeric intermediate is supported by a recent report of an IntI1 mutant with improved recombination rate and which is only slightly affected in the binding to the recombination sites [18].
We then isolated a mutant with improved utilization of fatty acid and designated it FitnessOle.
We also constructed and screened a random mutagenesis library and obtained one TtAG mutant with improved thermostability, which was analysed using site-directed mutagenesis and 3D structure modelling.
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We designed and proposed two high-throughput screening schemes for selecting l-threonine aldolase mutants with improved properties.
Here, we present a method that identifies protein mutants with improved overall cellular efficacy, an objective not feasible with previous approaches.
This approach allows the selection of mutants with improved stability, a parameter of great interest in any biotechnological application of photosynthetic proteins spanning from space research to biosensoric and photovoltaic fields, including biofuel and nutraceuticals production [44].
Computational strain design methods evaluate the effects of gene or reaction deletions to search for the mutants with improved production capabilities.
The use of recombinant HRP mutants with improved stability behavior (e.g. Morawski et al. 2001; Ryan and O'Fágáin 2007a), as well as upcoming studies thereon are likely to yield further beneficial contributions in the near future.
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