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Then, a mutant with higher glutaminase free binding energy was selected for further studies.
In this work, an evolutionary engineering approach was applied to select a spontaneous mutant with higher specific xylose consumption rate.
In this context, directed evolution of the α-l-arabinofuranosidase rPoAbf from Pleurotus ostreatus (Giacobbe et al. 2014) allowed us to develop an improved mutant with higher activity than the wild type enzyme.
This is because a successful somatic mutant with higher fitness relative to hematopoietic cells carrying the inherited allele has long survival and a long-term influence on the organism's fate.
The fact that 35S2∶MIR168b transformants with lower than wildtype AGO1 levels exhibit a lower 21/22 ratio than wildtype plants whereas the mir168a-2 mutant with higher than wildtype AGO1 levels exhibits a higher 21/22 ratio than wildtype plants suggests that preferential stabilization of miR168 by AGO1 only affects the 21-nt miR168 species but not the 22-nt species.
These substitutions resulted in a mutant with higher activity and stability for use in washing powders, for instance.
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The high number of NOE restrains, together with completeness of resonance assignments, allowed us to determine the structure of Q212P mutant with high resolution (Figure 2 and Table 1).
Transformation of the triple mutant with high-copy number plasmids containing any of the other eight genes encoding putative DGC and PDE proteins (Table 1) did not change the biofilm-negative and nonpigmented phenotype of the mutant (Fig. 2C and data not shown).
This group had previously characterized a lithium resistant Dictyostelium mutant, with high levels of IP3 and lacking the PREP gene [ 19].
The single mutant with highest selective advantage (called S83F) contributed 1.3% more offspring per generation than the parent strain, and the most competitive double mutant (S83F-D87N, whash has two changes within the gyrA gene) contributed 7.4% more offspring per generation.
We developed this mutant based on some published observations: first, single amino-acid mutation in Ng in the phenylalanine residue (F37) to tryptophan rendered Ng to a mutant with high affinity to CaM in the presence of Ca2+ (Gerendasy et al, 1994a).
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