The spectroelectrochemical cell contains 16 mL of suspension of a Chlamydomonas reinhardtii mutant with an appropriate mediator (2,6-DCBQ).
In order to isolate REL2 by map-based cloning strategy, a genetic population was constructed by crossing rel2 mutant with an indica rice cultivar, Kasalath.
Recently, we used a T-DNA insertion to identify a rice 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) mutant with an albino phenotype.
Since KNI-764 binds to the wild type with extremely high affinity, even after a 26-fold decrease, it still binds to the resistant mutant with an affinity comparable to that of other inhibitors against the wild type.
The isolate (Zool-3i) with a better enzyme activity (1.38 IU/ml) was subjected to induced mutagenesis using methyl methane sulphonate (MMS) and a mutant with an enzyme activity of 32.06 IU/ml was obtained.
A reverse genetic screen was used to identify a mutant with an insertion in HYDA2, followed by mutagenesis of the hydA2-1 strain coupled with a H2 chemosensor phenotypic screen to isolate the hydA1-1 hydA2-1 mutant.
Four human NSCLC cell lines were employed, expressing either of the following forms of the EGFR: (i) the wild-type receptor (QG56 cells), (ii) a mutant with an exon 19 in-frame deletion (HCC827 cells), (iii) a mutant with the exon 21 L858R point mutation NCI-H32555 cells), and (iv) a double mutant harboring the L858R and T790M mutations NCI-H19755 cells).
In addition, a mutant with an insertion in a gene encoding the type 1 fimbriae regulatory protein was also identified.
Two EMS mutants isolated 30 years ago, T-DNA mutants with complex insertion sites, and a mutant with an atypical, embryo-specific phenotype were resolved.
