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This allowed the detection of one mutant, which produced a 74% increase in hydrolysis compared to the parental enzyme.
Another M. tuberculosis mutant which produced a reduced level of SOD using antisense-sodA was sensitive to killing by hydrogen peroxide which led to attenuation of the mutant in mouse lungs and spleen [37].
This is in line with observations for the stm6 mutant, which produced 5-13 fold more H2 compared to the wild type [ 48].
Surprisingly, a Δ rpoS deleted E. coli mutant, which produced less extensive biofilms, was compromised in its ability to mount a LSMMG-induced resistance to the general stressors but not to the antibiotics.
The data were consistent with the spo0A mutant of C. beijerinckii[ 21], but different from the C. acetobutylicum spo0A mutant, which produced 75% less ethanol than WT cells [ 22].
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Because an equal amount of 21-nt and 22-nt miR168 species is observed in 35S2∶MIR168b transformants in which miR168 is predominantly produced by the 35S2∶MIR168b transgene (Figure 3A), the mir168a-2 mutant, which produces miR168 only from the MIR168b locus, was expected to produce an equal amount of 21-nt and 22-nt miR168 species.
We used the cpi1-1 mutant, which produces cyclopropylsterols as main sterol components (Men et al, 2008) and two smt1 mutants that accumulate cholesterol above wild-type levels (Schrick et al, 2002; Willemsen et al, 2003).
The 35S- TcANS transgene was also introduced into the Arabidopsis ans (ldox) T-DNA mutant, which produces hypocotyls that appear white to light green due to lack of anthocyanins, and seeds that appear light yellow due to lack of PAs.
The npr1-3 mutant, which produces a truncated cytoplasmatically localized npr1 protein with no nuclear localization signal, has been reported to be affected only in SA-dependent gene expression, not in JA and ET dependent genes.
A simple way to model this is to have the same low level of Hb activity as in the zygotic mutant, which produces a good prediction as just described.
Here we investigated the signals that mediate ER localization of AtGCSI, a type II membrane enzyme playing a key role in seed development, as shown by characterization of the GCSI Arabidopsis mutant which produces shrunken seeds where embryo development is blocked at the heart stage [ 42].
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