The exception in this region was the W36A mutant, which led to a 50% level in both Aβ species, possibly due to a change in the anchoring of the bottom of TMD1 with the lipid water interface and thereby allowing F37/38 to form part of the transmembrane domain.
We found that none of the single mutations had an observable effect on the MIC value of any substrate tested, whereas the maximum detrimental effect was observed for the triple mutant, which led to a 32-fold reduction of MIC for Nov and TPP.
The increased neddylation in the K472E/R473E Cul1 mutant, which leads to the disruption of a buried salt bridge in the four-helix bundomainmain of Cul1 [24], is likely a result of reduced binding of the deneddylating CSN complex (see Fig. 2b).
This reduction releases the suppression of the floral repressor FLC in hlp1-1 mutant, which leads to delayed flowering.
Among all the reported AF-linked Cx40 mutants, the Q49X mutant is the only nonsense mutant, which leads to a premature truncation of the Cx40 polypeptide.
We next determined whether TRF2ΔBΔM mutant, which leads to c-NHEJ-dependent telomeric fusions (Smogorzewska et al., 2002; van Steensel et al., 1998), is impaired for the interaction.
To test whether splicing factors are broadly required for RNA regulation, we took advantage of the fog-1 (q253ts ) mutant, which leads to failure of sperm production at the restrictive temperature of 25° but permits spermatogenesis at 15° (Barton and Kimble 1990; Jin et al. 2001a).
However, the use of the Tfm model to delineate the role of AR in Leydig cells is complicated both by the effects of the absence of AR in other cells in the testis and the hypothalamic-pituitary-gonadal axis, and also the impacts of cryptorchidism associated with the Tfm mutant, which leads to temperature-induced degenerative effects (23).
The T331A mutant was consistently more prone to open than WT and any other mutants, which led to the occasional simultaneous opening of several monomers.
These findings suggested that muscle cells are present and correctly localized but internally disorganized in y241 mutants, which led us to examine the subcellular architecture of myofibers.
Eveleigh prepared two separate lines of mutants which led to the hypercellulolytic strains RUT C30 and RL-P37 ([ 5]; Fig. 1), of which H. jecorina RUT C30 has become the most frequently used strain for laboratory cellulase production [ 6- 11].
