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Interestingly, we obtained a value of 1.67 for the cepR mutant, which indicates that this mutant is more competitive than K56-2 wild-type in the fruit fly (Figure 6C).
Positive interactions represent cases where the double mutant has a less severe phenotype than either single mutant, which indicates that the two network components should have different regulatory signs.
RyhB in the hfq-rnc-pnp mutant reached a similar amount of that in the hfq mutant, which indicates that PNPase was the main contributor in the degradation of Hfq-free RyhB [ 14].
Under LDs, the double mutant flowered significantly later than either single mutant, which indicates a complexity in the interaction between COP1 and phyB under these conditions that cannot be simply explained by regulation of CO protein levels (see Discussion).
However, after 48 hr, we see a more wild-type pattern in the msn2msn4 mutant, which indicates that the absence of Msn2 and Msn4 function delays, rather than prevents the production of R3 cells and the G1 arrest that is normally associated with quiescence.
Compared to the same anther developmental stage of the WT, many key genes involved in various aspects of anther development show a reverse gene expression pattern in the GMS mutant, which indicates that diverse gene regulation pathways are involved in the GMS mutant anther development.
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However, overexpression of the E. coli mpl gene on a multi-copy plasmid was shown to complement an E. coli thermosensitive murC mutant, which indicated that Mpl could also accept L-alanine as a substrate [13].
Although the swr1 mutant also showed sensitivity to DNA damaging agents, htz1 mutant grew slower than the swr1 mutant, which indicated that Htz1 may have Swr1-independent functions to regulate genome stability.
Furthermore, the chz1 nap1 double mutant also had increased levels of Htz1 on chromatin like the nap1 single mutant, which indicated that Nap1 may have a dominant role regulating Htz1 incorporation.
Many pollen grains were deeply stained in the WT, but no pollen grains were stained in the GMS mutant, which indicated that little nutritional materials were accumulated in the GMS mutant microspores.
Both BAGs and AIF had higher expression levels in the susceptible cultivar than in the resistant mutant, which indicated that PCD was increased by Foc TR4 attack in the wild-type, which conflicts with the low concentration of ROS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com