By contrast, all DFMs are formed and are arranged normally in lms null mutants, which implies that lms is not required for DFM muscle specification and morphogenesis.
The result suggests that these rice plants are likely homozygous or bi-allelic mutants, which implies that in this case the CRISPR/Cas may have completed the generation of DSBs in the first meristematic cell during regeneration of the rice plants from transgenic calli.
This is in agreement with findings from analysis of AsLOV2 mutants, which implied that electrostatic interactions are important for the photoresponse of the protein [ 20].
This is in contrast to the accelerated vegetative phase change observed in the Arabidopsis DCL mutants [7, 11], which implies that OsDCL4 has a broader role in development than the Arabidopsis DCL4.
In addition, diploid elg1 Δ srs2 Δ double mutants are dead, which implies that an essential function in the cell requires at least one of the two gene products for survival.
Furthermore, in-silico mutagenesis of the three models clearly indicates when the mutant state transitions are occurred, which implies changes in rate of gene expression between the wild-type and mutant conditions.
Thus local feedback enhanced a stable cycling of the same resources (i.e. indirect selection for a cycle of resources), which implies fewer mutants established in the populations and therefore a slow down of the evolutionary process.
Interestingly, we found that under full induction, the nuclear concentration of GFP-GFP-Cln3* is positively correlated and G1 length is negatively correlated with birth size, which implies that size control is maintained with the mutant Cln3 (Di Talia et al., 2007).
As observed in the ruv mutants, the RecG pathway does not result in linear DNA, which implies that no Holliday junction cleavage-ligation can be detected.
Based on its functional annotation, this gene encodes a protein involved in auxin-mediated signaling, which implies a late induction of auxin responsive genes in the mutant.
