Intriguingly, Wei et al. could show that the sch9 long-lived deletion mutant, which generates less superoxide, produces and exports glycerol under aerobic growth conditions when glucose or ethanol are available, thus generating a CR-like environment [ 49].
Given the nature of the deletion in the Col3a1▵ mutant, which generates a predicted null allele, along with its mixed genetic background, the most obvious interpretation for this discrepancy is the presence of genetic modifiers that increase the penetrance of aortic dissection in the +/ Col3a1▵ mutant when compared with the knockout mouse.
The functional consequence of hypertonia-associated Trak1 mutation, which generates a Trak1 mutant protein truncated at amino acid 824 (Fig. 3A), remains unknown.
For group 2 mutants, which generated smaller whole-cell currents (Fig. 1B), we noticed either reduced (D1275N, E161K) or unchanged (T220I) cell surface signals when compared to wild-type hNav1.5 (Fig. 6).
Interestingly, in a significant population of cells expressing R521G and R524S mutants, which generate only very small pool of cytoplasmic protein and rarely form visible aggregates, Pur-alpha and TIAR relocalize in SGs.
Careful analysis of the dynamics of dbx1 expression in wild-types and apoptosis in ablated mutants indicated that the EM subset, which generates a small subpopulation of CNCCs, is crucial to maintain the survival of neighbouring tissues.
There are also 15 class mutation operators which generate mutants which are warning insensitive.
Moreover, assuming an incremental testing strategy when combining static and dynamic analysis, this set of mutation operators which generate warning insensible mutants should be used during testing once the chance the faults they modeled be detected during automatic static analysis is reduced.
Unlike the EP/GPEET mutant, which was generated entirely in the bloodstream form [20], Δprocyclin did not show defects in differentiation from the bloodstream to the procyclic form, nor did it undergo growth arrest shortly after differentiation (data not shown).
The titre of this auxotrophic mutant, which was generated using classical mutagenesis, was only 2 g/L in batch culture [ 35].
pCEFL-HA- MAPK15, and all its deletion mutants were already described except for 1 300 MAPK15 deletion mutant which was generated by cloning the cDNA, obtained by PCR amplification, in the pCEFL-HA vector.
