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Overall, the transcriptomic analysis reveals a marked enhancement of the expression of genes involved in iron scavenging in the hemA mutant when treated with CORM-3.
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Multiple interactors were confirmed by testing the corresponding mutant strains when treated with ain-1 or ain-2 RNAi (Supplementary file 3).
However, ribavirin-resistant mutants emerged when treated with low concentrations (≤0.2 mM) of ribavirin, and those mutants were genetically more stable during serial passages in cell culture.
Hence, we checked the impact of WT KCNE1 on mutant R228C channels when treated with Cu-Phen.
Many interactions were confirmed by testing candidate mutants for phenotypes when treated with ain-1 and ain-2 RNAi (Supplementary file 3).
This view is supported by the findings that (i) the infection phenotype of lin-7 mutants was suppressed when treated with hsf-1 dsRNA, and (ii) loss of lin-7 resulted in a significant delay in DAF-16::GFP nuclear localization.
In addition, studies in NIH3T3 cells showed that a pAKT308 mutant failed to activate when treated with growth factor, while a pAKT473 mutant showed reduced activity; thus dual phosphorylation appears to be required for full AKT kinase activity 38.
The ain-1 (lf ) single mutant shows enhanced defects when treated with ced-3, ced-4, or egl-1 RNAi for two RNAi generations.
Likewise, lfy fil double mutants formed inflorescence pins when treated with a low dose of NPA.
We noted a tendency for injection of any of the eya1 mutants to slow otic development when treated embryos were compared with non-treated siblings at stage 32.
First, while cenp-metaΔ mutant cells arrested in mitosis when treated with colchicine (as reflected by the increased number of mitotic cells seen in cenp-metaΔ mutant treated cells), the relatively high level of cells with PSCS revealed that these cells were not able to maintain a prolonged arrest when treated with spindle poisons.
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