Sentence examples for mutant were transfected from inspiring English sources
pGL3, pGL3-MLL4, or pGL3-MLL4 G4 mutant were transfected in Aven−/− HEK293T cells with pRenilla, as a control for transfection efficiency, along with either pcDNA3.1, FLAG-Aven, FLAG-AvenΔRGG, or FLAG-AvenR-K.
The plasmids expressing EBV-Z, K-RTA and its mutant were transfected into 293T cells, and the interaction between EBV-Z and the mutant K-RTA was examined.
Wild-type Luc-2036 and its mutant were transfected into HepG2 cells in the presence or absence of PGC-1α and/or ERRα.
(A ) HT1080 cells stably expressing BST2 WT or the Y6/8A mutant were transfected to express Vpu or the ELV/AAA mutant.
p3XFlag CMV10 HIF-1α 652–826 or C800S mutant and HIF-2α 625–870 and C844S mutant were transfected for 3 h using FuGENE 6 (Roche) and then treated with −/+T-hydro for a further 3 h before collection.
The human RCC cell lines CAKI-2 (wild-type von Hippel-Lindau [VHL]) and RCC11 (a VHL mutant) were transfected with a nontargeting, control, short interfering RNA (siRNA) (5′-CATGCCTGATCCGCTAGTC-3′) or carbonic anhydrase 9 (CA9) siRNA (5′-GAGGAGGATCTGCCCAGTGAA-3′) (Qiagen, Manchester, UK).
Each mutant was transfected in cells with or without Cdh1 or Cdc20.
Results in Fig. 6B show that while NPM/B23 WT interferes with centrosome reduplication (25% of the cells exhibited the reduplication phenotype, i.e. >4 centrioles), this effect was even more pronounced when the S4A mutant was transfected (18% of cells with >4 centrioles).
We found that wild-type p33ING1 increased CSIG protein stability after UV irradiation; this effect was abolished when the p33Δ142 194 deletion mutant was transfected.
This observation was not only true for a situation where wild-type p53 was transfected in a p53 mutant cell line, but also for the opposite situation, where p53 mutant was transfected in a wild-type p53 cell line.
This was also the case when p53 wild-type was introduced in a p53 mutant cell line (HT29 series) and when a p53 mutant was transfected in a p53 wild-type cell line (LoVo series).
