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Two proteins that were specifically downregulated in the mutant were the Rubisco small subunit chain PW9 (A.n. P26667) and the photosystem I reaction center subunit IV (A.n. P13194).
Two proteins that were specifically upregulated in the mutant were the oxygen-evolving enhancer protein 2 corresponding to the PSBP subunit of photosystem II (A.n. Q00434), and catalase-1 (CA.n1; A.n. Q00434).
In both cases, data from the mlh1 ∆ mutant were the same as with wild type, with 1.78% Ade+ transformants and 47.02% due to template switching, indicating that mismatch repair has no apparent role in template switching or chromosomal recombination during BIR.
To our knowledge, the final titer (131.5 g/L), the productivity (0.84 g/L/h), and the yield (0.44 g/g crude glycerol) of 2,3-BDO from crude glycerol obtained by the double deletion mutant were the highest recorded in 2,3-BDO production from glycerol as the sole carbon source to date.
Moreover, the inability of SPL15:Splantsplanto to restore most of the WT phenotype as did 35S SPL15m + lines suggested that the enhanced seed carotenoid level and morphological traits observed in the sk156 mutant were the collective result of miR156 suppression of at least some other SPL genes rather than SPL15 alone and that SPL15 functions redundantly with some other SPL gene products.
Double-stranded RNA structure, as well as A-to-I RNA editing, is known to modulate alternative splicing (Nishikura, 2010), so we asked whether transcripts with altered intronic dsRNA in the mutant were the same transcripts with altered splicing in poly(A) RNA.
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This mutant is the first mutant of AChE capable of hydrolysing organophosphates.
Delayed melanin production in the pmt4 mutant was the only alteration of classical virulence-associated phenotypes.
The aos mutant was the one described in [ 15].
One such genetic mutant is the rpd3Δ:: KANMX strain.
The pfk2Δ mutant was the only mutant displaying a drop in NADH levels after glucose addition.
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