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Strains IMT5155Δyqi and IMT5155Δyqi (pDSK602 yqi), a strain carrying a modified expression plasmid with the yqi gene, that is, the complemented mutant, were tested against IMT5155, the positive control, and IMT11327, a negative control strain of avian origin lacking the gene yqi.
Multiple mutant versions of Cas9, including Cas9D10A, Cas9H840A, and a Cas9D10A,H840A double mutant, were tested.
The growth properties of each mutant were tested by spotting serial dilutions of galactose-grown cells on media containing fructose or glucose as the carbon source.
Two independent isolates of the deletion mutant were tested to ensure that the phenotypes observed were not due to off-target effects.
(C ) Similar to (B ), except that Flag-NEMO WT and UBD mutant were tested for their ability to interact with TRAF2 and MAVS.
To determine the importance of the dimerization of Nup53 for membrane tubulation the RRM mutants of fragments that contained either both (93 320 RRM mutant) or only the C-terminal binding site (130 320 RRM mutant) were tested.
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The tir1-1 afb5afb5 plants were crossed and the resulting homozygous double mutant was tested for dicamba resistance.
Further, each mutant was tested for its binding energetics with respect to the two substrates, dephosphocoenzyme A and ATP.
In the current study, the TEM8 L56A mutant was tested for its ability to block intoxication and was found to be very similar to CMG2.
Each Nse3 mutant was tested for its ability to interact with both Nse1 and Nse4 using the yeast two-hybrid system.
Each mutant was tested in a functional assay that measured FSHβ-luciferase activity in a stably transfected pituitary gonadotrope cell line (−338 FSHβ-Luc) [59].
More suggestions(15)
variants were tested
transformants were tested
yes were tested
mutant were determined
mutant were analyzed
mutant were considered
mutant were overexpressed
mutant were associated
mutant were performed
mutant were repaired
mutant were done
mutant were exposed
mutant were used
mutant were soaked
mutant were assayed
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