Exact(2)
When conidial suspensions of mossd1 mutant were spotted onto the leaves of HvCEBiP-silenced plants, small brown necrotic flecks or blast lesions were produced but these lesions did not expand beyond the inoculation site.
To determine growth rate of the several mutant types on the selective plate, 100 cells of each mutant were spotted (on a 2-cm spot) above a lawn of parent (10) cells on selective lactose medium.
Similar(57)
The P GAL1 - RPS26Arps26bΔ strain transformed with different plasmids encoding eS26 mutants were spotted in 10-fold dilutions on selective glucose containing plates and grown at indicated temperatures for 3 7 days.
A dilution series for the indicated mutants was spotted onto YE6S plates and YE6S with the indicated concentration of MMS, bleomycin or bleocin.
Wild type and mutant strains were spotted on the same chitin agar plate.
Serial dilution of mutant cells were spotted onto YPD plates containing 0 and 10 µg/ml concentrations of TBZ, together with the control hypersensitive α1-tubulin mutant, nda2[29].
In order to study the contribution of LhrA and Hfq to the chitinolytic activity of L. monocytogenes, bacterial suspensions of the wild type, ΔlhrA and Δhfq mutant strains were spotted on LB agar plates supplemented with chitin (Figure 5B).
To that end, wild-type H2B and the H2B K37 mutant strains were spotted on media containing the agents hydroxyurea (HU, an agent which blocks replication leading to replication fork collapse) or methyl methanesulfonate (MMS, an alkylating agent that causes DNA lesions and ultimately DNA strand breaks).
Strains containing the indicated WT and mutant alleles were spotted in 10-fold serial dilutions on 5-FOA-SD or SD and grown at 20 30°C for 3 6 days.
Wild-type cells and vps51Δ, vps52Δ, vps53Δ, and vps54Δ mutant cells were spotted on control plates and plates containing increasing concentrations of myriocin, as indicated.
RIO2 shuffle yrb2∆ strain transformed with the indicated combinations of empty, WT or mutant plasmids were spotted in 10-fold dilutions on SD-plates containing 5-FOA and grown at 25°C for 2 4 days.
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