It is also thought that the inactivation recovery of p.Arg583Gln mutant is slower than normal, leading to accumulation of many inactivated calcium channels during depolarization.
Root elongation of the mutant was slower than that of the wild-type in the absence of Al (WT, 40 ± 6.8 mm/48 h vs ral1, 25 ± 3.7 mm/48 h).
Previous investigations by Nakajima and Ueda ([1997]) and Bernát et al. ([2009]) showed that the growth rate of a Synechocystis ∆apcE mutant was slower than that of the wild-type strain under photoautotrophic conditions with 1% CO2, which corresponded with our results (Figure 4).
Although we evaluated Al resistance based on relative root elongation and root growth of ral1 mutant was slower than that of WT under control condition, we do not think that less root growth without Al treatment is necessarily correlated with higher relative root elongation under Al.
Namely, the rate of retinal release of the basal mutant is slower than that of WT.
In comparison to agr, sigB and hla mutants (see Figs. 6 and 7), the rate of intracellular killing of the aur mutant was slower, with live staphylococci consistently present in cell lysates up to day 3. Also, macrophages infected with a sortase A mutant, which is deficient in multiple MSCRAMMs, cleared bacteria more slowly (Fig. 8B) at a rate that was comparable to that of the aur mutant.
Analyzing hydrolytic products via TLC also showed that the release of smaller xylo-oligosaccharides by the deletion mutant was slower than in the wildtype.
When grown on this model, the bcr1/bcr1 mutant was slow in forming a mature biofilm, and after 24 hours of growth formed a biofilm comprised mainly of yeast cells, in contrast to its reference and complemented strains (Fig. 3).
We propose that, at low temperature, the I707L mutant is slowed each time it encounters pairs of adenosines (or possibly purines generally) due to the blockage of the active site by the template DNA.
Results of this experiment indicated that GALT3 (gand3-1 and galt3-2) and GALT6 (gand6-1 and galt6-2) mutands, and to a lesser extent the GALT4 (galt4-1 and galt4-2) mutants were slow to reorient their root growth compared to WT when grown in the presence of 100 mM NaCl.
