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Some secondary branches of the gns4 mutant were significantly shortened, which caused spikelet clustering.
In the current analysis, the promoters of the up/downregulated genes in the rad mutant were significantly enriched with common putative cis-elements which are connected to several TFs such as MYB, bZIP, ERF and ARF.
We transfected Agt5 −/− MEF cells with wild-type p62 or a LIR deletion mutant, which was reported to mediate its interaction with LC3 for autophagic degradation, and found that the protein level of p62 or its LIR deletion mutant were significantly reduced when parkin is expressed in these cells (Fig. 3C).
At later time points during the nymphal feeding, the numbers of bba64 mutant were significantly more abundant inside the engorged tick than of WT (Figure 2).
However, both phenotypes of the ΔlicD2 mutant were significantly attenuated for nasopharyngeal colonization with respect to the respective wild type counterpart (P<0.001 in both cases).
However, bacterial titers recovered from kidneys on day 7 post-infection of mice infected with the CFT073 ΔyheF ΔhofQ double mutant were significantly lower (p = 0.013) compared to WT CFT073 (Fig. 4B).
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The cell number in vascular tissue of pear sextuple mutant is significantly reduced before the onset of phloem PSE differentiation and activation of the phloem transport (see Supplementary Information).
The thermo-stability of the NHaseM (βW47E) mutant was significantly strengthened, while its activity reduced by nearly 50%.
The production of several secondary metabolites in R55 mutant was significantly improved using high-performance liquid chromatography (HPLC) analysis.
Interestingly, the frequency of mutations introduced by gRNA complementary to the deletion mutant is significantly higher than with gRNA complementary to WT donor.
Fecal shedding of the pnp mutant was significantly reduced during the 7-day study compared to the wild-type strain (p < 0.001).
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