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To identify SSR markers that could be used for molecular mapping, the TL-2 line and CS mutant were screened for polymorphisms at several SSR loci on each chromosome (data not shown).
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An original spl5 mutant was screened from a γ-radiation-mutagenesis population of Norin8 (Oryza sativa L. ssp. japonica) by Iwata et al. (1978) in Japan.
In this study, multi-tillering dwarf1 (mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7.
The positive mutant was screened by measuring the decrease in the absorbance of NADPH at 340 nm and high performance liquid chromatography (HPLC).
This vector was transformed into C. acetobutylicum DSM1731, and the CAC1502 disrupted mutant was screened according the reported procedure [30].
After 10 successive transfers in RCM medium in the absence of erythromycin (equivalent to 33 generations), a ctfAB-deleted mutant was screened from 1998 clones and designated as DDC1458.
Each mutant was screened for AHL-responsive luciferase activity by patching into two adjacent wells of a 96-well plate filled with motility agar containing either AHL (oxoC6) or the solvent control, acidified ethyl acetate (EA).
The crinkly leaf mutant was screened from our Hedou 12 gamma ray irradiated mutagenesis population.
Each mutant was screened at least twice and the results were scored as described in the Methods.
To verify that the isolated yeast was indeed a sub-strain of the parental recombinant D5A+ strain the isolated mutant was screened for resistance against the fungicide genetecin.
Finally, three types of LiP mutants were screened.
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