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Of the 22 SNPs, all three possible genotypes i.e. homozygous wild-type, heterozygous and homozygous mutant were present for 15 of them.
The parent Aichi/1968 NP and its N334H mutant were present at comparably high levels, but levels were markedly reduced for variants carrying each of the three individually deleterious mutations.
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If at this time, an m-hit mutant is present, this reduction in average fitness will allow it to be selected for and to quickly become the dominant population (Figure 3).
Interestingly, a greater amount of the R28D mutant was present in the supernatant.
These data confirmed that the chromophore group, missing in the knock-out mutant, is present in the complementation.
After the first photo-bleaching, virtually none of the GFP-fluorescent dots that were normally observed could be visualized when ARF1-GTP locked mutant was present.
We have not explored if this mutant is present at wild-type levels in cells, or if it is active for RydC regulation of cfa in vivo.
By contrast with FLAG-α1b-adrenoceptor-eYFP, the FLAG-α1b-adrenoceptor TMI-TMIV-eYFP mutant was present only as the 75 and 160 kDa forms.
A lower ratio indicates that a particular labeled transposon mutant is present at lower abundance in a culture relative to the abundance of a genomic DNA sample.
A typographic error for the YXXΦ binding mutant is present in previous publications (Honing et al., 2005; Edeling et al., 2006; Motley et al., 2006).
As seen in Figure 3C, the p.N478D mtPAP also showed a similar pattern of co-localization, consistent with the mutant being present in MRGs.
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