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After this stage, no differences between the WT and the mutant were observed until maturity (Fig. 6f and g).
However, biphasic kinetics and antisense oligonucleotide response trends opposite those of the well-studied G11C mutant were observed suggesting that new alternative pairings with multiple registers, termed Alt X and Alt Y, had been created.
No significant differences in morphological changes between the single Δhns and the ΔhnsrecB double mutant were observed (data not shown).
Because no defects in appressorium development by the ΔpdeH mutant were observed on hydrophobic surface, the development of infectious hyphae within the host cells was examined using excised leaf sheaths.
Three significant differences between the WT and the ASN3 mutant were observed.
More dramatic changes in the chromatin structure of the core X mutant were observed towards the telomere.
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The dominant-negative activity of the LEF-1 mutant was observed when the cell line was transiently transfected with TOPFlash.
No additional effects on tri-methylation levels by the not4S/T5A mutant are observed in strains deleted for BUR2 (Figure 3B).
When added at the same time, a further ∼2-fold increase in repression mediated by the TRβΔAF2 mutant was observed.
The inhibition of toxicity of Cry1Aa, Cry1Ac and Cry1Fa toxins by Cry1Ab-D136N/T143D mutant was observed at sub-stoichiometric ratio.
Notably, partial growth of the trkH mutant was observed in CDM+, while on solid CDM+ medium, growth of the mutant was totally impaired.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com