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Appropriate wild type controls corresponding to the genetic background of each mutant were included.
The results demonstrate that our high-content image-profiling method efficiently identified genes from pathways related to cell wall biosynthesis, although neither fks1 nor fks2 mutant were included in the candidates.
L er and the ga5 mutant were included in all experiments.
Three genes that resided in this interval and showed differential expression between WT and mutant were included.
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The nonpolar ipaH9.8 mutant is included in the pWR100 collection, with and without tetRA, under the name " ipaH9.8ΔNterm".
Due to the previously described phenotype [ 11], the ccw12Δ mutant was included as a control for growth inhibition.
A total of eight dissociation experiments have been conducted for wild-type HLA B*08 01fos, and the T134K mutant was included in six of these experiments.
The C. jejuni flgBC mutant was included as a negative control; this mutant binds to the cells at levels similar to that of a C. jejuni wild-type strain but is deficient in Cia protein secretion and delivery (not shown).
The C. jejuni ciaC mutant was included as a control, as this mutant displays a significant reduction in cell invasion compared to a wild-type strain of C. jejuni.
The gi-2 mutant was included to test the level of FT expression in this mutant when grown in non-sterile conditions and compare it to the -DEX TG plants.
Unlike the WT protein, no initial plateau region was observed when the triple mutant was included in the zincon titration, yet 180 μM Zn II) was still required to saturate 140 μM zincon.
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