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Mice vaccinated with a single dose of the F. tularensis LVSΔguaA or LVSΔguaB mutant were fully protected against subsequent lethal challenge with the LVS parental strain.
The individual S303A and LZ4m HSF1 mutants promoted human HSF1-dependent yeast growth to a similar extent, though neither the LZ4m nor the S303 mutant were fully derepressed, as the S303A/LZ4m double mutant displayed enhanced human HSF1-dependent yeast growth (Figure 3A).
Single point mutations were introduced into wild-type or mutant cDNAs using the QuikChange® site-directed mutagenesis protocol (Stratagene) and the coding regions of each mutant were fully sequenced.
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Upon conjugation of pPW plasmid, V. cholerae O1 and O139 die, while the transconjugants of the ΔtoxRS mutant are fully viable.
In our hands, a sap1 3Δ mutant was significantly attenuated while a sap4 6Δ mutant was fully virulent.
This mutant was fully functional in the oncogenic transformation rescue assay (Figure 2B).
As reported previously [13], the EDAA Nef mutant was fully active and Δ12-39 Δ12-39anddisplayE4A4ntermedisplayedintermediateG2activityxA Nef mutants whiledefective for this activity.
These data indicate that the gliding apparatus of the T192 mutant is fully functional and suggest that the increased ratio of non-motile cells observed is not caused by defects in the gliding mechanics.
Moreover, the OmpU G325D) mutant was fully virulent in a single round, competitive infection in infant rabbits.
Despite being apparently excluded from lipid rafts, the AC8 N-glycosylation-deficient mutant is fully responsive to CCE (Fig. 4 D ).
It needs be noted that Hellal-Levy et al. [ 16] found that the H950A MR mutant is fully active in the presence of aldosterone.
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CEO of Professional Science Editing for Scientists @ prosciediting.com