Sentence examples for mutant were extracted from inspiring English sources
Total RNA samples at different fiber developmental stages from 13 25 DPA in TM-1 and the im mutant were extracted using the CTAB-sour phenol extraction method [ 69].
For DGE sequencing, RNAs from rice leaves inoculated with the WT or ∆fliC mutant were extracted to prepare two cDNA libraries for RNA-seq analysis.
Total RNAs of S. mobaraensis ECU7480 and its mutants were extracted after cultivation for different times.
The plasmids in the 74 mutants were extracted and transformed into fresh BL21 DE3) cells for a double-check (Fig. 4C).
Gene IDs within the deleted chromosomal regions of the insertion mutants were extracted using Geneious version 7.1.5 created by Biomatters available from http://www.geneious.com.
Phospholipids of the harvested wild-type and fat-1 mutants were extracted with Folch method [ 38], and they were analyzed using LC-ESI-MS/MS with a 4000Q-TRAP triple quadrupole linear ion trap mass spectrometer (AB SCIEX, Framingham, MA, USA) equipped with an ACQUITY ultra-performance liquid chromatography system (Waters, Milford, MA, USA).
LPS from R mutants was extracted with phenol-chloroform-light petroleum (2∶5∶8) (165 mg of freeze-dried bacteria/ml) [65].
Total RNA from the testes of P44 mice (5 wild-type and 7 mutants) was extracted with the Trizol reagent.
Genomic DNA from C. glutamicum transposon mutants was extracted using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Taufkirchen, Germany).
Then, plasmid DNA for the 19 most active mutants was extracted and retransformed into naïve yeast for detailed analysis.
Total DNA of the isolates and mutants was extracted and purified from the mycelium by using the cetyltrimethylammonium bromide (CTAB) protocol (He 2000).
