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Roots of WT and the mutant were exposed to 20 μM Al for 24 h and then stained with 0.025 % Evans blue.
To determine Al content in roots, seedlings of WT and ral1 mutant were exposed to a 0.5 mM CaCl2 solution containing 20 μM Al for 6 h.
Seedlings of WT and ral1 mutant were exposed for a 0.5 mM CaCl2 solution (pH 4.5) containing 0, 20 μM Al, 5 μM Cd, 5 μM La for 48 h.
For a dose response experiment, 8 seedlings each of WT (cv. Kasalath) and ral1 mutant were exposed to a 0.5 mM CaCl2 solution containing 0, 10, 20, 50 or 100 μM Al Cl3 at pH 4.5 for 48 h.
To investigate whether the ral1 mutant had altered expression of Al-resistance genes, roots of WT and the mutant were exposed to 0 or 20 μM Al for 6 h and the expression of Al-resistance genes was determined.
Five-day-old seedlings of WT and ral1 mutant were exposed to a 0.5 mM CaCl2 solution (pH 4.5) containing 20 μM Al for 24 h, and then the roots were stained with 0.025 % Evans blue or 0.1 % Eriochrome Cyanine R for 10 min.
Similar(51)
The already elevated levels of AMPKαSsp2 T189 phosphorylation did not increase further over a 30-min period when the ptc1,2,3.Δ mutant was exposed to nitrogen stress.
When the imbibed seeds of atku80 atradoubledouble mutant, and the atku80 mutant, are exposed to γ-rays (100 Gy, 3.25 Gy/minute; source: Cs137), both mutants exhibit a similar acute hypersensitivity phenotype.
Therefore, the slightly elevated SA levels in unchallenged cycam1 suggest that [Ca2+]cyt elevation restricts SA accumulation, which becomes harmful if the mutant is exposed to SA-stimulating biotic and abiotic stress.
In contrast, it appeared that the regulation of anaerobic energy metabolism and protein degradation modules was largely Fur-independent, as very similar sets of genes also were identified when a fur mutant was exposed to iron depletion and repletion conditions [ 25].
The plate was heated for 10 min using the gradient method of an Eppendorf Mastercycler gradient PCR machine, with the gradient set at either 33 45°C or 46 58°C (each mutant was exposed to both of these gradients), the machine on the BLOCK setting, and the heated lid set to 75°C with the lid WAIT option.
More suggestions(15)
mutant were normalized
mutant were summarized
mutant were performed
mutant were analyzed
mutant were expressed
mutant were grown
mutant were isolated
mutant were generated
mutant were obtained
mutant were soaked
mutant were analysed
mutant were interpreted
mutant were done
mutant were produced
mutant were selected
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com